High throughput Single Cell Metagenomic Sequencing with Semi-Permeable Capsule: Unraveling Microbial Diversity at the Single-Cell Level in Sewage and Feces Microbiomes
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Isolating Microbial Cells
in Semi-Permeable Capsules
Product
Single-Cell Microbe Barcoding Kit for Genomic Analysis
Discover
Single-Cell Microbial WGS kit barcodes up to 10,000 microbial single amplified genomes (SAGs) per experiment, enabling genome recovery for individual cells after sequencing. Start by encapsulating microbial cells from suspension into semi-permeable capsules (SPCs), then seamlessly proceed with lysis, genome amplification, barcoding and library preparation. Optimized for Illumina short-read sequencing, the kit can also be adapted to any commercial NGS platform, including short- and long-read sequencing.
Features
HIGH THROUGHPUT
Up to 10,000 cells in a single experiment
RESOLUTION
Achieve strain-level taxonomic resolution
LINKAGE
Link individual genomes with extrachromosomal DNA
ASSEMBLY
Assemble single microbe genomes de novo
QUANTIFICATION
Quantify absolute microbial abundance
COST EFFICIENCY
Retrieve whole genomes for <1$ per cell (incl. sequencing)
Publications Using our Single-Cell Microbial WGS Kit
Kit components
Cell Lysis
- 2x Lysis Buffer
- Neutralization Buffer
Buffers
- 50x Wash Buffer
- 10x Stop Buffer
- Water
Debranching & End Prep
- Debranching Enzyme
- 10x Debranching Buffer
- End Prep Enzyme Mix
- End Prep Reaction Buffer
Whole Genome Amplification
- WGA Polymerase
- WGA Enhancer
- 10x WGA Reaction Buffer
- dNTP Mix
- Primer Mix
Barcoding
- Ligation Enzyme
- 10x Ligation Buffer
- Barcodes Plates
Library Preparation
- Fragmentation Enzyme Mix
- Fragmentation Reaction Buffer
- Ligation Adapter
- Ligation Master Mix
- Ligation Enhancer
- Amplification Master Mix
- Indexing Primer Mix
Specifications
| Catalog number | CKP-BARK8 |
| Sufficient for | 2 runs x 10,000 barcoded microbial cells |
| Sample type | Microbial cell suspension |
| For use with (equipment) |
|
| Sequencing | Illumina MiSeq, NextSeq 550, NovaSeq |
| Shipping conditions | Dry Ice |
| Storage | -20 °C |
| Intended Use | Research Use Only |
SAMPLE TYPES
Designed for versatility, our technology seamlessly integrates with diverse microbiomes and isolates, provided that the cells are in aqueous suspension and free from large particulates (>40 μm).
Soil
Marine
Human
Isolates
Wastewater
Other
White paper: Sample preparation for single-microbe isolation in semi-permeable capsules
Read moreLysis
SPCs can withstand harsh lysis conditions, which can be carried out in multiple steps. While the kit includes an alkaline lysis buffer, researchers have the flexibility to use their preferred lysis method, with the exception of mechanical disruption.
Chemical
Harsh detergents, Alkaline solutions
Enzymatic
Proteinase K, Lysozyme
Temperature
Up to 100℃
Selective Lysis
Compatible with commercial kits for host cell lysis
Mechanical
Not compatible with beat beating or other mechanical methods
MULTIPLEXING CAPABILITIES
Samples can be multiplexed at various stages of the workflow, maximizing efficiency.
Applications
Microbiome Profiling
Identify microbial strains within complex communities at high throughput for accurate insights into microbiome composition and functions.
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Microbial Dark Matter Discovery
Uncover hidden microbial diversity and functional potential by assembling genomes of uncultivated microbes de novo.
Virulence & Resistance
Monitor the spread of virulence factors and antimicrobial resistance genes via HGT by linking microbial genomes with extrachromosomal DNA.
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Virology
Perform de novo viral genome assembly in environmental samples and link viruses to hosts to uncover ecological dynamics and interactions.
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PROOF OF CONCEPT SEQUENCING SERVICE
Thinking about doing single-cell microbial genomics but not sure whether Single-Microbe DNA Barcoding kit will be a good fit?
Test your sample's full potential quickly and easily by sending it to us. The proof of concept experiment includes the following:
- Sample preparation
- Cell encapsulation into SPCs
- Single-microbe DNA barcoding
- Sequencing on the Illumina platform (NextSeq 550 or NovaSeq)
- Data demultiplexing by cellular barcodes
ADVANCED DATA ANALYSIS
We've partnered with the Single Cell Genomics Center at Bigelow Laboratory to offer advanced bioinformatics services for microbiologists lacking data analysis resources
Learn more
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