PRODUCT
SPC Innovator kit
Redefine Single-Cell Analysis and Develop Your Own Multi-Step Workflows
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SPC Innovator Kit is designed specifically for robust cell isolation into semi-permeable capsules (SPCs). Once encapsulated, multiple sample processing steps can be efficiently carried out through simple pipetting, ensuring the compartmentalization of cells and nucleic acids. Due to the distinct non-droplet nature of SPCs, each step in the workflow can be conducted under optimal conditions. This eliminates limitations in stability, inhibition, and the number of workflow steps. SPC Innovator Kit makes developing ultra-high-throughput single-cell assays easier than ever before.

Procedure
Encapsulation
Innovator Kit
Bulk-like
multi-step processing
WASH CYCLE
Remove buffer
Add wash reagents
Spin down
ADDITION CYCLE
Remove buffer
Add reagents
INCUBATION CYCLE
Spin down
Repeat the cycles
Detection
Sequencing
Growth
Storage
Features
Physical properties
Permeability

Retained: cells, >500bp DNA & RNA, particles.

Permeable to: proteins (enzymes, antibodies), small molecules (nucleotides, detergents, salts), oligonucleotides.

Capsules sealed in oil prevent small molecule leakage.

Chemical stability

Compatible with harsh lysis reagents.

Compatible with pH 2.0-13.0.

Compatible with cell fixation reagents.

Thermal stability

Compatible with heating up to 100 °C.

Compatible with storing at 4 °C (aqueous solution) and -80 °C (alcohol solution).

Mechanical stability

Robust (particle-like).

Compatible with pipetting, centrifuging and sonication.

Mild release

Contents can be released at room temperature.

Release reagent is biocompatible and does not affect cells or nucleic acids.

Capabilities
Biocompatibility
Eukaryotic Cells
Adherent, suspension
Bacterial Cells
Yeast Cells
Algal Cells
Molecular Biology
Isothermal Amplification
Thermal Cycling
Reverse Transcription
Transposition
Ligation
Nucleic Acid Modification
Sequencing
Single-cell Labeling
Short-read Platforms
Long-read Platforms
Enrichment
Stain
Cells
Nucleic Acids
Retain small molecules
SORT
FACS
Sort fluorescent cells and nucleic acids
Dispense
EXCITED TO ENABLE
Sequencing
Single-cell DNA-seq
Single-cell ATAC-seq
Single-cell multiomic sequencing
Immune profiling
Targeted sequencing
Microbiology
Antimicrobial resistance surveillance
Cultivation of anaerobic microorganisms
Extremophiles research
Viral genomics
Other
Spheroid formation
Nuclei isolation
Nucleic acid cytometry
Co-culturing
Custom
No reagent lock-in for your custom single-cell assay development
Kit components
Consumables
  • Syringes & Safe-Needles
  • Collection Tubing
  • Disposable Hemocytometer
Chip
  • Co-flow Microfluidic Chip
Reagents
  • Core Reagent
  • Shell Reagent
  • Photoinitiator
  • Sample Loading Oil
  • Capsule Stabilization Oil
  • Emulsion Breaker
  • Wash Additive
  • Release Reagent
Specifications
Catalogue number
CKN-G11
CKN-G52
Compatible with FACS
No
Yes
Size
60 – 70 μm
30 – 35 μm
Sample type
Eukaryotic and prokaryotic cells, nucleic acids, particles
Eukaryotic (up to 10 μm) and prokaryotic cells, nucleic acids, particles
Sufficient for 6 runs
Shipping conditions Ambient
Storage 4 °C
Intended use Research Use Only
WHITE PAPER Multistep workflows within microcompartments Application Note Increasing throughput of single-bacterial cell analysis by SPC size reduction APPLICATION NOTE Barcoding-free multiplex single-cell amplicon-seq APPLICATION NOTE High-throughput single-cell cultivation in semi-permeable capsules APPLICATION NOTE Guidelines for successful in-capsule PCR Application Note High-throughput analysis and enrichment of semi-permeable capsules (SPCs) using FACS DOCUMENT Product Catalogue
Start with SEMI-PERMEABLE CAPSULE GENERATION
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