Styx High-Throughput Screening Platform
Styx

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HIGH-THROUGHPUT SCREENING PLATFORM

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Styx is a high-throughput screening platform built to address the growing need for advanced functional screening in complex biological research. It seamlessly integrates multicolor fluorescence analysis, pressure pumps, and easy-to-use software into an elegant and compact design to bring you the most robust and advanced fluorescence-activated droplet sorting (FADS) device on the market.

Styx Droplet Sorting Overview
Styx Multicolor Fluorescence Detection

Technical Note: High-throughput screening powered by microfluidics-based droplet sorting

STYX COMPONENTS

Styx Open Wireframe
Styx Sorter Open View

Pressure pumps

Styx Pressure Pumps

Four integrated pressure pumps ensure precise droplet reinjection, offering accurate flow control with rapid response rates — all without the need for extra components.

4 Lasers & Detectors

Styx 4 Lasers & Detectors

Four integrated lasers (405nm, 488nm, 561nm, and 637nm) and independent wide-band detectors, create a cohesive system for efficient fluorescence excitation and accurate capture. This integration allows for a diverse range of fluorophores used in functional assays.

Forcefield Generator

Styx Forcefield Generator

Facilitates droplet sorting with minimal configuration, featuring two independent channels that can operate in tandem or in distinct pulsed and continuous modes, enabling versatile workflows involving combinations of merging and sorting.

Automated Stage

Styx Automated Stage

Securely anchors microfluidic chips during experiments and ensures accurate chip positioning for droplet detection and imaging.

Microscope Illuminator

Styx Microscope Illuminator

Provides controlled, adjustable infrared illumination, enhancing visibility and contrast in the selection process without interference with commonly used fluorophores.

Magnetic Chip Adapter

Styx Magnetic Chip Adapter

Provides a user-friendly snap-on electrical connection to the chip, enabling sorting without the need for brittle electrode pins or fickle liquid electrodes.

Open

Styx Closed Wireframe
Styx Sorter Closed View

High-speed Camera

Styx High-speed Camera

Equipped with dual cameras, the system records and enables real-time observation of rapid droplet selection events at up to 4000 frames per second, concurrently capturing two independent areas for comprehensive monitoring.

Integrated Microscopy

Styx Integrated Microscopy

Dual-camera microscopy enables imaging and tracking of microfluidic sorting processes at multiple scales with 1080p resolution. The two imaging sites allow investigating mechanistic sorting details and particle outcomes in positive and negative outlets.

Embedded UI

Styx Embedded Touchscreen UI

Provides users with an intuitive and interactive platform to select sorting parameters for the desired droplet population across multiple platforms, without the need of software installation.

Chip Holder

Styx Chip Holder

Securely anchors microfluidic chips during experiments and ensures accurate chip positioning for imaging.

Closed

TOP OF THE LINE USER INTERFACE

Benefits

High Throughput

Rapid library screening with sorting rates up to
1000Hz

Functional Assays

Specific target selection based on activity

Flexibility

Compatible with many functional assays with a fluorescence readout

Convenience

Fully integrated platform with an intuitive user interface

Versatility

Multiparameter selection supported by 4 lasers and wide-band detectors

Compact Design

Compact, portable, and wireless-ready design for seamless operation

Workflow

Step 1: Droplets with fluorescent targets

Droplets with fluorescent targets

Step 2: Tubes with droplets and reagents loaded into Styx

Tubes with droplets and reagents loaded into Styx

Step 3: Droplets reinjected into microfluidic chip

Droplets reinjected into microfluidic chip

Step 4: Droplets are selected based on fluorescence

Droplets are selected based on fluorescence at speeds of up to 1000Hz

Step 5: Positive hits collected in tubes

Positive hits collected in tubes

DROPLET SELECTION SIMPLIFIED

Droplet selection, which relies on high-voltage, has been simplified with our snap-on electrical connection. The magnetic adapter ensures quick, accurate placement and directs voltage precisely to the necessary chip components.

High-Voltage Droplet Selection Module

STYX APPLICATIONS

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Start your workflow by encapsulating your library

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Onyx Library Encapsulation Platform

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